Workshops-iptal

*Please select two options below. These sessions are fully hands-on; participants will actively engage in experimental procedures
** Please select two options below. These sessions are non-hands-on, guided, and interactive. 

Program Structure
Hands-on Experimental Techniques

• Establishment of Ex vivo Cultures – Organoid-Based Systems
• Establishment of Ex vivo Cultures – Organotypic Culture Systems
• DRG isolation& Culture & 3D Migration Analysis
• Islet Isolation & Functional Assessment
• Data Interpretation for Translational Research (Omics-based)

Interactive Translational Sessions

• TIL Isolation and Interpretation
• Immune Profiling & Flow Cytometry
• Behavioral Tests in Pancreatic Disease Models
• Integrative Omics & MALDI Imaging
• AI-Assisted Pathology & Digital Tissue Analysis
• CAM Tumor Model for Rapid Functional Assessment
• Zebrafish-Based Tumor Assays: Live Imaging and Functional Readouts 

Session Details

Hands-on Experimental Techniques
Establishment of Ex vivo cultures – Organoid-Based Systems

• Organoid initiation from mouse tissue (including duct picking and enzymatic digestion)
• Seeding and dome formation (Matrigel handling and plating)
• Co-culture design and setup
• QC and troubleshooting

Establishment of Ex vivo Cultures – Organotypic Culture Systems

• Tissue slicing & handling
• Ex vivo culture setup
• Native microenvironment preservation
• Functional readouts & QC

DRG isolation& Culture & 3D Migration Analysis

• DRG isolation and culture
• Neurite outgrowth assessment
• 3D migration assay setup
• Live imaging and data interpretation

Islet Isolation & Functional Assessment

• Pancreatic digestion & islet separation
• Islet picking and purity assessment
• Viability & morphology scoring
• Basic functional readouts (stimulus/response logic)

Data Interpretation for Translational Research (Omics-based)

• Introduction to omics datasets used in pancreatic research
• Guided exploration of transcriptomic data
• Identifying biologically meaningful patterns
• Linking experimental design to omics output
• Common pitfalls in omics interpretation

Interactive Translational Sessions
TIL Isolation and Interpretation

• Tumor tissue dissociation for immune cell recovery
• Enrichment of tumor-infiltrating lymphocytes
• Viability and yield assessment
• Distinguishing true TILs from peripheral immune cells
• Translational considerations for downstream analyses

Immune Profiling & Flow Cytometry

• Principles of immune phenotyping
• Sample preparation for flow cytometry
• Marker selection and panel logic
• Data acquisition and basic gating strategies
• Common pitfalls and interpretation challenges

Behavioral Tests in Pancreatic Disease Models

• Rationale for behavioral testing in pancreatic disease
• Mechanical sensitivity assessment (von Frey test)
• Locomotor and anxiety-related behavior (Open Field test)
• Experimental design and sources of bias
• Interpretation of behavioral readouts and translational relevance

Integrative Omics & MALDI Imaging

• Overview of multi-omics approaches in pancreatic research
• Introduction to spatially resolved omics
• MALDI imaging: principles and sample requirements
• Linking molecular patterns to tissue architecture
• Strengths, limitations, and translational potential

AI-Assisted Pathology & Digital Tissue Analysis

• Whole-slide imaging and digital pathology workflows
• AI-Guided Tissue Analysis: Opportunities and Pitfalls
• Human–AI interaction: decision support vs automation
• Risks of over-interpretation in translational studies

CAM Tumor Model for Rapid Functional Assessment

• CAM preparation and windowing (demo + guided setup)
• Tumor cell implantation on CAM
• Fluorescent labeling for tumor visualization
• Assessment of tumor growth and angiogenic response
• Imaging-based interpretation of invasion and vascularization

Zebrafish-Based Tumor Assays: Live Imaging and Functional Readouts

• Tumor cell preparation and fluorescent labeling
• Embryo handling and tumor cell introduction (demo-guided)
• Live imaging of tumor growth and dissemination
• Functional readouts: invasion, angiogenesis, drug response
• Basic data interpretation and model limitations